Gus Goose is Donald Duck's second cousin, and the great-nephew of Grandma Duck. Gus's main personality trait is being quite gluttonous, as Donald discovered the hard way when Gus came for a visit in Gus' first appearance, the 1939 animated short Donald's Cousin Gus. Within the Disney comics, Gus is usually shown as living on Grandma Duck's farm outside of Duckburg, where he works as her farmhand. Along with his gluttony, Gus is shown in the comics as being quite lazy, often doing little if any work on Grandma's farm. He also has a tendency of falling asleep at random occasions, sometimes even standing up. Gus Goose has appeared as boyfriend of a classy and rich anthropomorphic swan called Cissy Swann in Danish stories.

Duckula has been a vampire for centuries. He can only be destroyed by exposure to sunlight or by a wooden stake thrust through his heart. Duckula has died numerous deaths, but he always returns through a mystic ritual, performed once each century, "when the moon is in the eighth house of Aquarius." Dr. Von Goosewing is a mad scientist and vampire hunter, a spoof of Abraham Van Helsing. He is a goose that speaks in a German accent. Von Goosewing wears an outfit not unlike that of Sherlock Holmes, with a pair of spats. He often flies a dirigible with "VG" on it. He pursues Count Duckula relentlessly, never able to comprehend that Duckula is actually completely harmless.